Radicals scavenging assay. Fractions were subjected to in-vitro HepG2 cell line study. Additional, by far the most potent fraction (EAF) was subjected to in-vivo hepatoprotective possible against CCl4 challenged rats. The in-vivo hepatoprotective active fraction was chromatographed on silica column to isolate the bioactive constituent(s). Structure elucidation was completed by utilizing various spectrophotometric procedures like UV, IR, 1 H NMR, 13 C NMR and MS spectroscopy. Ethyl acetate fraction (EAF) of hydro-alcoholic extract of U. dioica possessed the potent antioxidant activity viz. DPPH (IC50 78.99 0.17 g/ml) and NO (IC50 101.39 0.30 g/ml). The in-vitro HepG2 cell line study showed that the EAF prevented the cell harm. The EAF substantially attenuated the increased liver enzymes activities in serum and oxidative parameters in tissue of CCl4 -induced rats, suggesting hepatoprotective and anti-oxidant action respectively. Column chromatography of most potent antioxidant fraction (EAF) bring about the isolation of 4-hydroxy-3-methoxy cinnamic acid (ferulic acid) which can be responsible for its hepatoprotective possible. Hence, the present study suggests that EAF of hydro-alcoholic extract has substantial antioxidant and hepatoprotective potential on CCl4 induced hepatotoxicity in-vitro and in-vivo. 2015 Published by Elsevier Ireland Ltd. This really is an open access post below the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Article history: Received 23 January 2015 Received in revised form 29 July 2015 Accepted 30 July 2015 Offered on the net three August 2015 Key phrases: Urtica dioica Linn. Antioxidant HepG2 cell line Hepatoprotective Ferulic acid1. Introduction Liver is among the essential organ of our body and plays a very important function within the maintenance, efficiency and regulating homeostasis of our physique [45]. Liver issues have develop into on the list of really serious overall health difficulties as well as a key result in of morbidity and mortality around the globe. Practically 20,000 deaths and 250,000 new circumstances happen to be reported every year [42]. The percentage of liver toxicity as a consequence of several exposures is substantially larger in establishing countries like India (80 ) when compared with sophisticated countriesAbbreviations: UD, Urtica dioica; PEF, petroleum ether fraction; EAF, ethyl acetate fraction; NBF, n-butanol fraction; AF, aqueous fraction; SGOT, serum glutamate oxaloacetate transaminase; SGPT, serum glutamate pyruvate transaminase; ALP, alkaline phosphatase; CCl4 , carbon tetrachloride; MDA, malondialdehyde; CAT, catalase; GSH, glutathione; OD, optical density; HepG2, human hepatocellular carcinoma cells.Buy6-Fluoroquinoline-2-carbaldehyde Corresponding author.Oxetane-3-carbaldehyde manufacturer Tel.PMID:24101108 : +91 9915 939996; Fax: +91 1870 250002. E-mail address: ankalia [email protected] (A.N. Kalia).(two ) [51]. Oxidative strain plays a major function within the development of liver illnesses. The liver injury is initiated by the different toxic agents created by chemicals, alcohol, viruses or by their bio-activation to chemically reactive metabolites. These metabolites is often free of charge radicals, which either elicits an immune response or directly affects the biochemistry of the cells by interacting with cellular macromolecules. Even following the advancement in modern day system of medicine, there is absence of a trustworthy synthetic liver protective drug. Therefore, all-natural extracts /products from medicinal plants are regarded to become safe and efficient for the treatment of liver disorders [62]. The plants would be the rich source of bioactive compounds viz. organic polyphenols in addition to a numb.