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Mar. Drugs 2013, 11, 42794293; doi:10.3390/mdOPEN ACCESSmarine drugsISSN 16603397 www.mdpi.com/journal/marinedrugs ArticleEfficient Screening of Marine Extracts for Protease Inhibitors by Combining FRET Based Activity Assays and Surface Plasmon Resonance Spectroscopy Primarily based Binding AssaysTony Christopeit 1,two,, Kersti erb, U. Helena Danielson 2 and Inge W. NilsenNofima AS, Muninbakken 913, Troms291, Norway; EMails: kersti.overbo@nofima.Eugenol acetate site no (K.Price of 3-(tert-Butyl)cyclohexanone ); ingew.PMID:23489613 [email protected] (I.W.N.) Department of ChemistryBMC, Uppsala University, Box 576, Uppsala 751 23, Sweden; Email: [email protected] Author to whom correspondence must be addressed; E mail: [email protected]; Tel.: 4777629234. Received: 3 July 2013; in revised kind: 20 October 2013 / Accepted: 21 October 2013 / Published: 30 OctoberAbstract: The screening of extracts from marine organisms is really a extensively employed method to uncover new drug leads. A frequent difficulty inside the screening process is definitely the generation of false optimistic hits by way of unspecific effects in the complicated chemical composition on the crude extracts. In this study, we explored a combination of a fluorescence resonance power transfer (FRET) based activity assay along with a surface plasmon resonance (SPR) based binding assay to avoid this issue. An aqueous extract was ready from rest raw material in the Norwegian spring spawning herring, and further fractionated by methanol solubility and solid phase extraction. FRET primarily based activity assays had been used to figure out the influence of every single extract around the activity of differ.
